Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6249
Title: Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia
Authors: Mileski, Ksenija 
Ćirić, Ana D.
Gašić, Uroš M.
Žarković, Lazar 
Krivošej, Zoran
Džamić, Ana 
Marin, Petar 
Keywords: antimicrobial;antioxidant;BSA;enzyme inhibitory activity;Laserpitium siler;LC-MS;phenolics;Pseudomonas aeruginosa
Issue Date: 1-May-2023
Journal: Records of Natural Products
Volume: 17
Issue: 3
Start page: 453
End page: 475
Abstract: 
The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant, antimicrobial, anti-denaturation, acetylcholinesterase, tyrosinase, α-amylase, and α-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts, chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity, with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits, 95.08%-aerial parts, and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of α-glucosidase activity.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6249
ISSN: 1307-6167
DOI: 10.25135/rnp.364.2207.2528
Appears in Collections:Journal Article

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