Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/5939
Title: Applicability assessment of a standardized microsatellite marker set in endangered Busha cattle
Authors: Stevanov-Pavlović, Marija
Dimitrijević, Vladimir
Marić, Saša 
Radović, Dejan
Stevanović, Jevrosima
Stanimirović, Zoran
Keywords: Microsatellite;Paternity testing;Busa;Busha
Issue Date: 2015
Rank: M23
Publisher: Univ Ljubljana, Veterinary Faculty, Ljubljana
Journal: Slovenian Veterinary Research
Volume: 52
Issue: 3
Start page: 133
End page: 139
Abstract: 
This study is focused on evaluating 12 microsatellite markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225, BM1824) as recommended by International Society of Animal Genetics (ISAG) for paternity testing in Busha cattle in Serbia. A herd of 47 heads of Busha cattle were sampled for blood. The number of alleles ranged from 6 (ETH10) to 16 (TGLA122), with a mean value of 9.5. Total number of alleles at all 12 analyzed loci was 114. PIC (Polymorphism Information Content) values ranged from 0.513 (in BM1818) to 0.905 (TGLA53). Power of exclusion (PE) for single markers ranged from 0.228 (BM1818) to 0.607 (BM2113) and the power of discrimination (PD) from 0.75 (BM1818) to 0.96 (TGLA227). The combined power of exclusion and discrimination was very high (0.999 and 0.995 respectively) when all 12 markers were used in combination. Cervus software performed 96% successful paternity assignments. These results show that the 12 markers set recommended by I...
URI: https://biore.bio.bg.ac.rs/handle/123456789/5939
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