Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/5516
Title: Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae
Authors: Obradović, Mina
Malešević, Milka
Di Luca, Mariagrazia
Kekić, Dušan
Gajić, Ina
McAuliffe, Olivia
Neve, Horst
Stanisavljević, Nemanja
Vukotić, Goran 
Kojić, Milan
Keywords: Lastavirus;Lytic bacteriophage;Klebsiella pneumoniae;Tail fiber;Phage resistance
Issue Date: 25-Feb-2023
Rank: M22
Publisher: MDPI
Journal: Viruses
Volume: 15
Issue: 3
Start page: 628
Abstract: 
Klebsiella pneumoniae is a global health threat and bacteriophages are a potential solution in combating pandrug-resistant K. pneumoniae infections. Two lytic phages, LASTA and SJM3, active against several pandrug-resistant, nosocomial strains of K. pneumoniae were isolated and characterized. Their host range is narrow and latent period is particularly long; however, their lysogenic nature was refuted using both bioinformatic and experimental approaches. Genome sequence analysis clustered them with only two other phages into the new genus Lastavirus. Genomes of LASTA and SJM3 differ in only 13 base pairs, mainly located in tail fiber genes. Individual phages, as well as their cocktail, demonstrated significant bacterial reduction capacity in a time-dependent manner, yielding up to 4 log reduction against planktonic, and up to 2.59 log on biofilm-embedded, cells. Bacteria emerging from the contact with the phages developed resistance and achieved numbers comparable to the growth control after 24 h. The resistance to the phage seems to be of a transient nature and varies significantly between the two phages, as resistance to LASTA remained constant while resensitization to SJM3 was more prominent. Albeit with very few differences, SJM3 performed better than LASTA overall; however, more investigation is needed in order to consider them for therapeutic application.
URI: https://biore.bio.bg.ac.rs/handle/123456789/5516
ISSN: 1999-4915
DOI: 10.3390/v15030628
Appears in Collections:Journal Article

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