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Title: | Dynamics of pretreated wheat straw saccharification by cellulosome of Trichoderma viride | Authors: | Galić, M. Stajić, M. Ćilerdžić, J. |
Keywords: | Agricultural residues;Cellulases;Depolymerization;Biological pretreatment;Trichoderma viride | Issue Date: | 14-Oct-2022 | Rank: | M22 | Journal: | Cellulose Chemistry and Technology | Volume: | 56 | Issue: | 9-10 | Start page: | 1013 | End page: | 1019 | Abstract: | The main goal of this study was to analyze the potential of Trichoderma viride BEOFB 1210m for cellulase production during solid-state fermentation of wheat straw pretreated with the ligninosome of a well-known white-rot delignificator – Pleurotus pulmonarius HAI 573. After only 7 days of T. viridae BEOFB 1210m cultivation on biologically pretreated wheat straw, this micromycete produced the most active xylanases, which were also the dominant enzymes, with a value of even 3730.10 U L-1. Likewise, maximal but much lower values of exocellulases (155.83 U L-1) and βglucosidases (59.98 U L-1) were detected after the same period of fermentation. However, much higher activity of endocellulase (2439.55 U L-1) was obtained on the 10th day. The dynamics of enzyme activity was reflected on the level of substrate depolymerization. As much as 30.56% of the cellulose was degraded already on the 7th day, and that percentage did not change significantly until the end of the cultivation period. A significant loss of hemicelluloses was also measured at the beginning of the fermentation process, but it reached a maximum of approximately 50% by the 21st day. The results clearly showed that the selected T. viride strain has very good potential to synthesize highly active cellulases when grown on a cheap and available substrate, which is significant for further large-scale industrial applications. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/5459 | ISSN: | 0576-9787 |
Appears in Collections: | Journal Article |
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