Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/5274
Title: In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
Authors: Jovanović Marić, Jovana
Kolarević, Stoimir 
Đorđević, Jelena 
Sunjog, Karolina
Nikolić, Ivan 
Marić, Ana 
Ilić, Marija
Simonović, Predrag 
Alygizakis, Nikiforos
Ng, Kelsey
Oswald, Peter
Slobodnik, Jaroslav
Žegura, Bojana
Vuković-Gačić, Branka 
Paunović, Momir
Kračun-Kolarević, Margareta
Keywords: Genotoxic potential;Alburnus alburnus;Joint Danube Survey 4;Comet assay;Weight-of-evidence approach
Issue Date: 11-Nov-2022
Rank: M22
Publisher: Oxford Academic
Journal: Mutagenesis
Abstract: 
Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
URI: https://biore.bio.bg.ac.rs/handle/123456789/5274
ISSN: 0267-8357
DOI: 10.1093/mutage/geac024
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