Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/5274
Title: | In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study | Authors: | Jovanović Marić, Jovana Kolarević, Stoimir Đorđević, Jelena Sunjog, Karolina Nikolić, Ivan Marić, Ana Ilić, Marija Simonović, Predrag Alygizakis, Nikiforos Ng, Kelsey Oswald, Peter Slobodnik, Jaroslav Žegura, Bojana Vuković-Gačić, Branka Paunović, Momir Kračun-Kolarević, Margareta |
Keywords: | Genotoxic potential;Alburnus alburnus;Joint Danube Survey 4;Comet assay;Weight-of-evidence approach | Issue Date: | 11-Nov-2022 | Rank: | M22 | Publisher: | Oxford Academic | Journal: | Mutagenesis | Abstract: | Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/5274 | ISSN: | 0267-8357 | DOI: | 10.1093/mutage/geac024 |
Appears in Collections: | Journal Article |
Show full item record
SCOPUSTM
Citations
1
checked on Nov 1, 2024
Page view(s)
5
checked on Nov 4, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.