Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/5074
Title: Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations
Authors: Radonjić, Mia
Petrović, Jelena
Milivojević, Milena
Stevanović, Milena 
Stojkovska, Jasmina
Obradović, Bojana
Keywords: Tumor engineering;Alginate hydrogel;Perfusion bioreactor;Mathematical modeling;Glioma C6 cell line;Embryonic teratocarcinoma;NT2/D1 cell line
Issue Date: Sep-2022
Rank: M23
Publisher: Association of the Chemical Engineers of Serbia
Citation: Radonjić Mia, Petrović Jelena, Milivojević Milena, Stevanović Milena, Stojkovska Jasmina, Obradović Bojana (2022):Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations Chemical Industry and Chemical Engineering Quarterly 28(3) pp. 211-223
Project: Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. 451-03-9/2021-14/200135, Grant no. 451-03-9/2021-14/200287 and Grant no. 451-03-9/2021-14/200042
Journal: Chemical Industry and Chemical Engineering Quarterly
Abstract: 
A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the
same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 μ m s-1 induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse
effects of fluid shear stresses estimated as ∼67 mPa. On the contrary, similar velocity (100 μ
m s-1) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion
indicated that the medium partially flows through the hydrogel (interstitial velocity of ∼10 nm s-1). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers.
Substances with diffusion coefficients of ∼10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (∼10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
URI: https://biore.bio.bg.ac.rs/handle/123456789/5074
ISSN: 1451-9372
2217-7434
DOI: 10.2298/CICEQ210607033R
Appears in Collections:Journal Article

Show full item record

SCOPUSTM   
Citations

1
checked on Nov 1, 2024

Page view(s)

10
checked on Nov 3, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.