Different Levels of EGF, VEGF, IL-6, MCP-1, MCP-3, IP-10, Eotaxin and MIP-1훼 in Adipose Derived Stem Cells (ADSCs) Secretome in Androgenetic Alopecia

Abstract

BACKGROUND Hair follicles and underlying adipose tissue show highly coordinated interaction through exchange of different signaling molecules. We conducted a study to investigate the quantitative and qualitative secretome profiling of Adipose Derived Stem Cells (ADSCs) from different zones of hair growth in patients with Androgenetic Alopecia (AGA).METHODS We included 6 male patients, candidates for follicular unit extraction hair transplantation, all in early stage of AGA. 1mm punch samples of adipose tissue located beneath hair follicles of 3 scalp areas (alopecia, border-line and normal hair growth) and 1 periumbilical sample were analyzed.Samples were enzymatically digested, centrifuged, washed, and cell pellets were ceded and maintained in culture medium until reached monolayer. Conditioned media samples were thawed and analyzed with 41plex kit. Results were registered by Magpix device (Luminex platform) and calculated with xPonent software. We analyzed the levels of 35 signaling proteins.RESULTSThe level of Inteleukin-6 (IL-6) were significantly higher in the alopecia zone in comparison to the periumbilical and occipital, alongside with the levels of Vascular Endothelial Growth Factor (VEGF), Endothelial Growth Factor (EGF) and Eotaxin. The similar trend was found for Monocyte Chemotactic Protein-3 (MCP-3), Interferon gamma-inducible Protein-10 (IP-10) and Macrophage Inflammatory Protein - 1 alpha (MIP-1𝛼), while, on the other side, Monocyte Chemoattractant Protein-1 (MCP-1) level was the lowest in alopecia comparing to other zones. All other examined proteins did not shown changes in their expression level.CONCLUSION Hence, knowledge of differences in these signaling molecules expression will be essential for both, achieving therapeutic goals for hair loss conditions and shading more lights on the AGA etiology.