Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4507
Title: In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents
Authors: Devrnja, Nina
Krstić-Milošević, Dijana
Janošević, Dušica 
Tešević, Vele
Vinterhalter, Branka
Savić, Jelena
Ćalić, Dušica.
Keywords: Tansy;In vitro cultivation;Essential oil;Methanol extracts;Histochemical analysis;Phytochemical analysis
Issue Date: 27-Nov-2020
Rank: M21
Publisher: Springer
Journal: Protoplasma
Volume: 258
Issue: 3
Start page: 587
End page: 599
Abstract: 
In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.
URI: https://biore.bio.bg.ac.rs/handle/123456789/4507
ISSN: 0033-183X
1615-6102
DOI: 10.1007/s00709-020-01588-9
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