Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4393
Title: Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit
Authors: Chatzopoulou, Fani
Sanmartin, Maite
Mellidou, Ifigeneia
Pateraki, Irini
Koukounaras, Athanasios
Tanou, Georgia
Kalamaki, Mary S.
Veljović-Jovanović, Sonja
Cvetić Antić, Tijana 
Kostas, Stefanos
Tsouvaltzis, Pavlos
Grumet, Rebecca
Kanellis, Angelos K.
Keywords: Cucumis melo;Ascorbate oxidase;Ascorbic acid;Ripening;Ethylene
Issue Date: Nov-2020
Rank: M21
Citation: Fani Chatzopoulou, Maite Sanmartin, Ifigeneia Mellidou, Irini Pateraki, Athanasios Koukounaras, Georgia Tanou, Mary S. Kalamaki, Sonja Veljović-Jovanović, Tijana Cvetić Antić, Stefanos Kostas, Pavlos Tsouvaltzis, Rebecca Grumet, Angelos K. Kanellis, Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit, Plant Physiology and Biochemistry, Volume 156, 2020, Pages 291-303
Journal: Plant Physiology and Biochemistry
Abstract: 
Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.
URI: https://biore.bio.bg.ac.rs/handle/123456789/4393
ISSN: 0981-9428
DOI: 10.1016/j.plaphy.2020.08.040
Appears in Collections:Journal Article

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