Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4317
Title: Polarization-resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy
Authors: Miler, I
Rabasović, MD
Aleksić, Marija 
Krmpot, AJ
Kalezić, A
Janković, A
Korać, Bato 
Korać, Aleksandra 
Issue Date: Mar-2021
Rank: M21
Publisher: Wiley
Journal: J Biophotonics
Volume: 14
Issue: 3
Start page: e202000362
Abstract: 
Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two-photon excited fluorescence (TPEF) on 5 μm thick skin paraffin sections from 15-day-, 1-month- and 21-month-old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization-resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the β coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label-free method to previously fixed.
URI: https://biore.bio.bg.ac.rs/handle/123456789/4317
DOI: 10.1002/jbio.202000362
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