Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4272
Title: A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
Authors: Oalđe, Mariana 
Kolarević, Stoimir 
Živković, Jelena
Alimpić-Aradski, Ana 
Jovanović Marić, Jovana
Kračun Kolarević, Margareta
Đorđević, Jelena 
Marin, Petar 
Šavikin, Katarina
Vuković-Gačić, Branka 
Duletić-Laušević, Sonja 
Issue Date: 12-Mar-2021
Rank: M21
Publisher: Royal Society of Chemistry
Citation: Oalđe, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpić Aradski, Ana, Jovanović Marić, Jovana, Kračun Kolarević, Margareta, Đorđević, Jelena, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka and Duletić-Laušević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro", "Food Funct..", 2021, vol. 12(7), pg. 3233-3245
Journal: Food & Function
Abstract: 
This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts’ radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g−1), naringin (up to 5.07 mg g−1) and luteolin-7-O-glucoside (up to 3.54 mg g−1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
URI: https://biore.bio.bg.ac.rs/handle/123456789/4272
ISSN: 2042-6496
2042-650X
DOI: 10.1039/D1FO00447F
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