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Title: | The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe | Authors: | Wallace, Megan A Coffman, Kelsey A Gilbert, Clément Ravindran, Sanjana Albery, Gregory F Abbott, Jessica Argyridou, Eliza Bellosta, Paola Betancourt, Andrea J Colinet, Hervé Eric, Katarina Glaser-Schmitt, Amanda Grath, Sonja Jelić, Mihailo Kankare, Maaria Kozeretska, Iryna Loeschcke, Volker Montchamp-Moreau, Catherine Ometto, Lino Onder, Banu Sebnem Orengo, Dorcas J Parsch, John Pascual, Marta Patenkovic, Aleksandra Puerma, Eva Ritchie, Michael G Rota-Stabelli, Omar Schou, Mads Fristrup Serga, Svitlana V Stamenković-Radak, Marina Tanaskovic, Marija Veselinovic, Marija Savic Vieira, Jorge Vieira, Cristina P Kapun, Martin Flatt, Thomas González, Josefa Staubach, Fabian Obbard, Darren J |
Keywords: | DNA virus;Endogenous;viral element;Drosophila,;Nudivirus;Galbut virus;Filamentous virus;Adintovirus;Densovirus;Bidnavirus | Issue Date: | 2021 | Rank: | M21 | Citation: | Citation Tools The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe Megan A. Wallace, Kelsey A. Coffman, Clément Gilbert, Sanjana Ravindran, Gregory F. Albery, Jessica Abbott, Eliza Argyridou, Paola Bellosta, Andrea J. Betancourt, Hervé Colinet, Katarina Eric, Amanda Glaser-Schmitt, Sonja Grath, Mihailo Jelic, Maaria Kankare, Iryna Kozeretska, Volker Loeschcke, Catherine Montchamp-Moreau, Lino Ometto, Banu Sebnem Onder, Dorcas J. Orengo, John Parsch, Marta Pascual, Aleksandra Patenkovic, Eva Puerma, Michael G. Ritchie, Omar Rota-Stabelli, Mads Fristrup Schou, Svitlana V. Serga, Marina Stamenkovic-Radak, Marija Tanaskovic, Marija Savic Veselinovic, Jorge Vieira, Cristina P. Vieira, Martin Kapun, Thomas Flatt, Josefa González, Fabian Staubach, Darren J. Obbard bioRxiv 2020.10.16 | Journal: | Virus Evolution | Abstract: | Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA viruses detected in a metagenomic analysis of approximately 6500 pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new Nudiviruses, a new and divergent Entomopox virus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of Galbut virus, a dsRNA Partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a Bidnavirus, may be composed of up to 12 segments and represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2% or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/4264 | ISSN: | 2057-1577 | DOI: | 10.1093/ve/veab031 |
Appears in Collections: | Journal Article |
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