Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3996
Title: High dietary fructose load aggravates lipid metabolism in the liver of Wistar rats through imbalance between lipogenesis and fatty acid oxidation
Authors: Teofilović, Ana
Bursać, Biljana
Djordjevic, Ana
Vojnović Milutinović, Danijela
Matić, Gordana 
Veličković, Nataša
Keywords: Dietary fructose;fatty acid oxidation;lipogenesis;liver;rat
Issue Date: 2016
Journal: TURKISH JOURNAL OF BIOLOGY
Series/Report no.: 40;1235-1242
Abstract: 
Fructose ingestion is often associated with hepatic steatosis and hypertriglyceridemia. The homeostasis of hepatic lipids is
mainly determined by the interplay of lipogenesis and fatty acid β‑oxidation. In this study, we hypothesized that high fructose intake
disturbs hepatic lipid metabolism through an imbalance between these processes. Therefore, we analyzed the effects of a 9-weeklong
consumption of a 60% fructose solution on physiological parameters, glycemia, and blood lipid profiles in male Wistar rats. The
expression of key regulators of fatty acid oxidation (FAO) and lipogenesis in the liver were assessed by western blot and quantitative
polymerase chain reaction. The results showed that fructose-fed rats were normoglycemic and hypertriglyceridemic with visceral
adiposity, but without hepatic lipid deposition. A high-fructose diet is associated with increased nuclear levels of the lipogenic regulator
sterol regulatory element binding protein 1c (SREBP-1c), which was followed by increased acetyl‑CoA carboxylase and fatty acid synthase
mRNAs. The nuclear level of the FAO transcriptional regulators peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1)
and lipin‑1 were unaltered, while carnitine palmitoyltransferase 1 (CPT1) mRNA was significantly decreased. Overall, our findings
showed that liquid fructose overconsumption is associated with perturbation of hepatic lipid metabolism through predominance of
lipogenesis over β-oxidation, resulting in spillover of triglycerides and visceral adiposity.
URI: https://biore.bio.bg.ac.rs/handle/123456789/3996
ISSN: 13000152
13036092
DOI: 10.3906/biy-1512-40
Appears in Collections:Journal Article

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