Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3744
Title: New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: phytochemical analysis, cytotoxicity and antimicrobial activity
Authors: Dimkić, Ivica 
Petrović, Marija
Gavrilović, Milan 
Gašić, Uroš
Ristivojević, Petar
Stanković, Slaviša 
Janaćković, Peđa 
Keywords: Centaurea calcitrapa;Phytochemistry;Liquid chromatography–mass spectrometry;Metabolite profle;New antimicrobials;Cytotoxicity
Issue Date: 2020
Rank: M22
Journal: AMB Express
Abstract: 
Ethnobotanical and ethnopharmacological studies of many Centaurea species indicated their potential in folk medicine so far. However, investigations of diferent Centaurea calcitrapa L. extracts in terms of cytotoxicity and antimicrobial activity against phytopathogens are generally scarce. The phenolic profle and broad antimicrobial activity (especially towards bacterial phytopathogens) of methanol (MeOH), 70% ethanol (EtOH), ethyl-acetate (EtOAc), 50% acetone (Me2CO) and dichloromethane: methanol (DCM: MeOH, 1: 1) extracts of C. calcitrapa leaves and their potential toxicity on MRC-5 cell line were investigated for the frst time. A total of 55 phenolic compounds were identifed: 30 phenolic acids and their derivatives, 25 favonoid glycosides and aglycones. This is also the frst report of the presence of centaureidin, jaceidin, kaempferide, nepetin, favonoid glycosides, phenolic acids and their esters in C. calcitrapa extracts. The best results were obtained with EtOAc extract with lowest MIC values expressed in µg/mL ranging from 13 to 25, while methicillin resistant Staphylococcus aureus was the most susceptible strain. The most susceptible phytopathogens were Pseudomonas syringae pv. syringae, Xanthomonas campestris pv. campestris and Agrobacterium tumefaciens. The highest cytotoxicity was recorded for EtOAc and Me2CO extracts with the lowest relative and absolute IC50 values between 88 and 102 µg/mL, while EtOH extract was the least toxic with predicted relative IC50 value of 1578 µg/mL. Our results indicate that all tested extracts at concentration considered as non-toxic can be one of great importance in combat towards phytopathogenic and human pathogenic strains, as well as natural sources of antimicrobials
URI: https://biore.bio.bg.ac.rs/handle/123456789/3744
ISSN: 2191-0855
DOI: 10.1186/s13568-020-01120-5
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