Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3692
Title: Lipids status and copper in a single astrocyte of the rat model for amyotrophic lateral sclerosis: Correlative synchrotron‐based X‐ray and infrared imaging
Authors: Kreuzer, Martin
Stamenković, Stefan 
Chen, Si
Andjus, Pavle 
Dučić, Tanja
Keywords: X-ray fluorescence microscopy;amyotrophic lateral sclerosis;copper;lipids;synchrotron based Fourier transformed infrared microscopy
Issue Date: 2020
Rank: M21
Citation: Kreuzer M, Stamenković S, Chen S, Andjus P, Dučić T. Lipids status and copper in a single astrocyte of the rat model for amyotrophic lateral sclerosis: Correlative synchrotron-based X-ray and infrared imaging. J Biophotonics. 2020 May 28:e202000069. doi: 10.1002/jbio.202000069. Epub ahead of print. PMID: 32463554.
Journal: Journal of Biophotonics
Abstract: 
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease, causing death of motor neurons controlling voluntary muscles. The pathological mechanisms of the disease are only partially understood. The hSOD1-G93A ALS rat model is characterized by an overexpression of human mutated SOD1, causing increased vulnerability by forming intracellular protein aggregates, inducing excitotoxicity, affecting oxidative balance and disturbing axonal transport. In this study we followed the bio-macromolecular organic composition and compartmentalization together with trace metal distribution in situ in single astrocytes from the ALS rat model and compared them to the control astrocytes from nontransgenic littermates by simultaneous use of two synchrotron radiation-based methods: Fourier transform infrared microspectroscopy (SR-FTIR) and hard X-ray fluorescence microscopy (XRF). We show that ALS cells contained more Cu, which colocalized with total lipids, increased carbonyl groups and oxidized lipids, thus implying direct involvement of Cu in oxidative stress of lipidic components without direct connection to protein aggregation in situ.
URI: https://biore.bio.bg.ac.rs/handle/123456789/3692
ISSN: 1864-063X
1864-0648
DOI: 10.1002/jbio.202000069
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