Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3618
Title: Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells
Authors: Stojković, Dejan
Dias, Maria Inês
Drakulić, Danijela
Barros, Lillian
Stevanović, Milena 
C. F. R. Ferreira, Isabel
D. Soković, Marina
Keywords: O. spinosa;aerial parts;antibiofilm;antifungal;cytotoxicity;methanolic extract;phenolic compounds
Issue Date: Apr-2020
Rank: M21
Journal: Pharmaceuticals
Series/Report no.: 13(4);78
Abstract: 
Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
URI: https://biore.bio.bg.ac.rs/handle/123456789/3618
ISSN: 1424-8247
DOI: 10.3390/ph13040078
Appears in Collections:Journal Article

Show full item record

SCOPUSTM   
Citations

27
checked on Nov 2, 2024

Page view(s)

6
checked on Nov 3, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.