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Title: | Isolation and identification of Bacillus spp. From compost material, compost and mushroom casing soil active against Trichoderma spp | Authors: | Stanojević, Olja Milijašević-Marčić, Svetlana Potočnik, Ivana Stepanović, Miloš Dimkić, Ivica Stanković, Slaviša Berić, Tanja |
Keywords: | Agaricus bisporus;Bacillus;Biocontrol;Green mold;Hypervariant region | Issue Date: | 1-Jan-2016 | Rank: | M23 | Publisher: | Srpsko biološko društvo i grupa naučnih instituta | Project: | Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control Studies on plant pathogens, arthropods, weeds, and pesticides with a view to developing the methods of biorational plant protection and safe food production |
Journal: | Archives of Biological Sciences | Volume: | 68 | Issue: | 4 | Start page: | 845 | End page: | 852 | Abstract: | The isolation of bacteria was carried out from samples of straw and chicken manure, compost at various stages of the composting process and casing soil used for growing button mushrooms. A preliminary screening of 108 bacterial isolates for antagonistic activity against Trichoderma aggressivum f. europaeum showed that 23 tested isolates inhibited mycelial growth of the pathogenic fungus. Further screening with four indicator isolates of fungi revealed that all 23 bacterial isolates inhibited the growth of T. aggressivum f. europaeum, T. harzianum and T. koningii, while only 13 isolates inhibited the growth of T. atroviride. T. aggressivum f. europaeum proved to be the most sensitive, with many bacterial isolates generating a high percentage of growth inhibition. Only two bacterial isolates (B-129 and B-268) were successful in inhibiting the growth of all 4 tested pathogens. All 23 bacterial isolates were characterized as Gram-positive and catalase-positive and were subjected to molecular identification based on the partial sequence, the hypervariant region of the 16S rDNA. It was shown that the obtained bacterial strains belong to Bacillus subtilis, B. amyloliquefaciens, B. licheniformis and B. pumilus species. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/3243 | ISSN: | 0354-4664 | DOI: | 10.2298/ABS151104073S |
Appears in Collections: | Journal Article |
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