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Title: | Lactolisterin BU, a novel class II broad-spectrum bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 | Authors: | Lozo, Jelena Mirkovic, Nemanja O'Connor, Paula M. Malesevic, Milka Miljkovic, Marija Polovic, Natalija Jovčić, Branko Cotter, Paul D. Kojic, Milan |
Keywords: | Antilisterial activity;Bacteriocin;Lactolisterin BU | Issue Date: | 1-Nov-2017 | Journal: | Applied and Environmental Microbiology | Abstract: | © 2017 American Society for Microbiology. Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversedphase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/3181 | ISSN: | 0099-2240 | DOI: | 10.1128/AEM.01519-17 |
Appears in Collections: | Journal Article |
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Lozo et al_2017_AEM.pdf | Lozo et al_2017_AEM | 1.74 MB | Adobe PDF | Request a copy |
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