Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/26
Title: Involvement of PI3K, akt and RhoA in oestradiol regulation of cardiac iNOS expression
Authors: Zafirović, Sonja
Sudar-Milovanović, Emina
Obradović, Milan
Đorđević, Jelena 
Jasnić, Nebojša 
Borović Labudović, Milica
Isenović, Esma
Keywords: Cardioprotection;CVS;ENOS;Inducible nitric oxide synthase;Oestradiol;Rat heart
Issue Date: 1-Jan-2019
Rank: M22
Journal: Current Vascular Pharmacology
Abstract: 
© 2019 Bentham Science Publishers. Background: Oestradiol is an important regulatory factor with several positive effects on the cardiovascular (CV) system. We evaluated the molecular mechanism of the in vivo effects of oestradiol on the regulation of cardiac inducible nitric oxide (NO) synthase (iNOS) expression and activity. Methods: Male Wistar rats were treated with oestradiol (40 mg/kg, intraperitoneally) and after 24 h the animals were sacrificed. The concentrations of NO and L-Arginine (L-Arg) were determined spectro-photometrically. For protein expressions of iNOS, p65 subunit of nuclear factor-κB (NFκB-p65), Ras homolog gene family-member A (RhoA), angiotensin II receptor type 1 (AT1R), insulin receptor substrate 1 (IRS-1), p85, p110 and protein kinase B (Akt), Western blot method was used. Co-immunoprecipitation was used for measuring the association of IRS-1 with the p85 subunit of phos-phatidylinositol-3-kinase (PI3K). The expression of iNOS messenger ribonucleic acid (mRNA) was measured with the quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis of the tissue was used to detect localization and expression of iNOS in heart tissue. Results: Oestradiol treatment reduced L-Arg concentration (p<0.01), iNOS mRNA (p<0.01) and protein (p<0.001) expression, level of RhoA (p<0.05) and AT1R (p<0.001) protein. In contrast, plasma NO (p<0.05), Akt phosphorylation at Thr 308 (p<0.05) and protein level of p85 (p<0.001) increased after oestradiol treatment. Conclusion: Our results suggest that oestradiol in vivo regulates cardiac iNOS expression via the PI3K/Akt signaling pathway, through attenuation of RhoA and AT1R.
URI: https://biore.bio.bg.ac.rs/handle/123456789/26
ISSN: 1570-1611
DOI: 10.2174/1570161116666180212142414
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