Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/2197
Title: Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli
Authors: Vukotić, G. 
Strahinić, I.
Begović, J.
Lukić, J.
Kojić, M.
Fira, D. 
Keywords: casein;Lactobacillus;proteinases;prtP;prtR
Issue Date: 1-Jan-2016
Journal: Microbiology (Russian Federation)
Abstract: 
© 2016, Pleiades Publishing, Ltd. Lactocepins or CEPs are large cell wall bound extracellular proteinases of lactic acid bacteria, involved in protein breakdown and utilization. They are responsible for many health-promoting traits of food products fermented with these organisms, but also essential for probiotic effects of certain strains. Different mesophilic strains selected within the species Lactobacillus zeae, Lb. casei, Lb. rhamnosus, and Lb. plantarum were analyzed for their proteolytic activity towards main fractions of milk proteins—caseins and whey proteins. The strains showing excellent proteolytic features were further examined for presence of corresponding proteinase gene(s). It was found that Lb. zeae LMG17315 possessed catalytic domains of three distinct proteinase genes, unique feature in Lb. casei group, which are similar but not identical to previously characterized prtP and prtR genes. Lb. casei neotype strain ATCC393 was also analysed and based on obtained results its reclassification in taxon Lb. zeae is supported. In addition, we report catalytic domain of prtR-type gene in Lb. plantarum LMG9208, which is first such report in this species, and it is first time that this gene is reported outside Lb. casei group.
URI: https://biore.bio.bg.ac.rs/handle/123456789/2197
ISSN: 0026-2617
DOI: 10.1134/S002626171601015X
Appears in Collections:Journal Article

Files in This Item:
File Description SizeFormat Existing users please
Vukotic et al 2016. Microbiology.pdfVukotic et al 2016. Microbiology731.66 kBAdobe PDF
    Request a copy
Show full item record

SCOPUSTM   
Citations

27
checked on Nov 20, 2024

Page view(s)

2
checked on Nov 21, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.