Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/2173
Title: Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene
Authors: Andrejevic, Marko
Keckarević Marković, Milica 
Bursac, Biljana
Mihajlović, Milica 
Tanasic, Vanja
Kecmanović, Miljana 
Keckarević, Dušan 
Keywords: Cytochrome b;Forensic casework;Mitochondrial DNA;MT-CYB;Sequence analysis;Species identification
Issue Date: Jun-2019
Rank: M22
Journal: Forensic Science, Medicine, and Pathology
Abstract: 
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. The total number of analyzed species in this study was 30, including 17 mammalian and 13 bird species. Using a newly created primer pair, we successfully amplified and sequenced the target sequence in almost all tested species. The amplification was incomplete in just two species, and as a result, partial, but still variable sequences, were obtained. Using the target fragment we successfully identified all tested samples. Initial results suggested that the intraspecies genetic diversity of the target region, in all tested species, was low – from 0 to 4.72%. The interspecies genetic diversity of the target region, crucial for successful discrimination, showed relatively high diversity, ranging from 8.36% to 42.52%. Given its short length, the target region should be used for species determination, particularly in samples that are degraded or are low in DNA quantity.
URI: https://biore.bio.bg.ac.rs/handle/123456789/2173
ISSN: 1547-769X
DOI: 10.1007/s12024-019-00096-4
Appears in Collections:Journal Article

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