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Title: | Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” fish farm, Serbia | Other Titles: | Određivanje genetičke strukture potočne pastrmke (Salmo trutta) iz ribnjaka „Braduljica“, Srbija | Authors: | Molerović, Nikola Rašković, Božidar Đedović, Radica Ostojić Andrić, Dušica Marković, Zoran Marić, Saša |
Keywords: | Salmo trutta;CR mtDNA;LDH nDNA;PCR;RFLP;Selection | Issue Date: | 2019 | Rank: | M24 | Publisher: | Institut za stočarstvo | Project: | Improvement of production capacities of the carp (Cyprinus carpio L) using feeding and selective breeding programs | Journal: | Biotechnology in Animal Husbandry | Volume: | 35 | Start page: | 289 | End page: | 299 | Abstract: | Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/1867 | ISSN: | 1450-9156 | DOI: | 10.2298/BAH1903289M |
Appears in Collections: | Journal Article |
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Molerovic et al - 2019 - Braduljica.pdf | 510.51 kB | Adobe PDF | Request a copy |
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