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https://biore.bio.bg.ac.rs/handle/123456789/1539
Title: | Effects of vanadate on the mycelium of edible fungus Coprinus comatus | Authors: | Žižić, Milan Zakrzewska, Joanna Tešanović, Kristina Bošković, Eleonora Nešović, Milica Karaman, Maja |
Keywords: | Coprinus comatus;EPR;NMR;Phosphate metabolism;SEM;Vanadium | Issue Date: | 1-Dec-2018 | Journal: | Journal of Trace Elements in Medicine and Biology | Abstract: | © 2018 Elsevier GmbH Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. 31P NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. 51V NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/1539 | ISSN: | 0946-672X | DOI: | 10.1016/j.jtemb.2018.07.017 |
Appears in Collections: | Journal Article |
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Effects of vanadate on the mycelium of edible fungus Coprinus comatus.pdf | 559.24 kB | Adobe PDF | Request a copy |
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