Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1539
Title: Effects of vanadate on the mycelium of edible fungus Coprinus comatus
Authors: Žižić, Milan
Zakrzewska, Joanna
Tešanović, Kristina 
Bošković, Eleonora
Nešović, Milica
Karaman, Maja
Keywords: Coprinus comatus;EPR;NMR;Phosphate metabolism;SEM;Vanadium
Issue Date: 1-Dec-2018
Journal: Journal of Trace Elements in Medicine and Biology
Abstract: 
© 2018 Elsevier GmbH Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. 31P NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. 51V NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1539
ISSN: 0946-672X
DOI: 10.1016/j.jtemb.2018.07.017
Appears in Collections:Journal Article

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