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Title: | The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus | Authors: | Gačić, Zoran Kolarević, Stoimir Sunjog, Karolina Kračun-Kolarević, Margareta Paunović, Momir Knežević Vukčević, Jelena Vuković Gačić, Branka |
Keywords: | 5-Fluorouracil;Unio tumidus;Unio pictorum;Comet assay | Issue Date: | 1-Jan-2014 | Journal: | Environmental Pollution | Abstract: | The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 μM) with 0.4 μM being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 μM 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures. © 2014 Elsevier Ltd. All rights reserved. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/1440 | ISSN: | 0269-7491 | DOI: | 10.1016/j.envpol.2014.04.024 |
Appears in Collections: | Journal Article |
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