Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1440
Title: The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus
Authors: Gačić, Zoran
Kolarević, Stoimir 
Sunjog, Karolina
Kračun-Kolarević, Margareta
Paunović, Momir
Knežević Vukčević, Jelena 
Vuković Gačić, Branka 
Keywords: 5-Fluorouracil;Unio tumidus;Unio pictorum;Comet assay
Issue Date: 1-Jan-2014
Journal: Environmental Pollution
Abstract: 
The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 μM) with 0.4 μM being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 μM 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures. © 2014 Elsevier Ltd. All rights reserved.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1440
ISSN: 0269-7491
DOI: 10.1016/j.envpol.2014.04.024
Appears in Collections:Journal Article

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