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Title: | Evaluation of genotoxic pressure along the sava river | Authors: | Kolarević, Stoimir Aborgiba, Mustafa Kračun-Kolarević, Margareta Kostić, Jovana Simonović, Predrag Simić, Vladica Milošković, Aleksandra Reischer, Georg Farnleitner, Andreas Gačić, Zoran Milačič, Radmila Zuliani, Tea Vidmar, Janja Pergal, Marija Piria, Marina Paunović, Momir Vuković Gačić, Branka |
Issue Date: | 1-Sep-2016 | Project: | Grant agreement 603629-ENV-2013-6.2.1. (Globaqua) - Implementation of Microbial Source Tracking (MST) method for assessment of faecal pollution in the Sava River and relation (potential relationship) to the presence of genotoxic agents |
Journal: | PLoS ONE | Abstract: | ©2016 Kolarević et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimuriumTA1535/ pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/ Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrialwastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples). |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/1392 | DOI: | 10.1371/journal.pone.0162450 |
Appears in Collections: | Journal Article |
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Kolarevic ea 16 Genotoxic pressure Sava.PDF | 3.32 MB | Adobe PDF | Request a copy |
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