Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/137
Title: Regulatory role of PGC-1α/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation
Authors: Stancic, Ana
Buzadzic, Biljana
Korać, Aleksandra 
Otasevic, Vesna
Jankovic, Aleksandra
Vucetic, Milica
Markelić, Milica 
Veličković, Ksenija 
Golić, Igor 
Korać, Bato 
Keywords: Cold acclimation;Metabolism;Skeletal muscle;Transcription control
Issue Date: 1-Nov-2013
Rank: M21
Project: White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders 
Journal: Journal of Experimental Biology
Abstract: 
This study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4±1°C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and Nω-nitro-Larginine methyl ester (L-NAME) treated. Compared with controls (22±1°C), there was an initial increase in the protein level of 5'- AMP-activated protein kinase a (day 1), followed by an increase in peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1a) and peroxisome proliferator-activated receptors (PPARs): PPARa and PPARγ from day 1 and PPARδ from day 7 of cold acclimation. Activation of the PGC-1α/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in β-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1α/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation. © 2013. Published by The Company of Biologists Ltd.
URI: https://biore.bio.bg.ac.rs/handle/123456789/137
ISSN: 0022-0949
DOI: 10.1242/jeb.089334
Appears in Collections:Journal Article

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