Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1343
Title: Genetic characterization of broodstock brown trout from Bled fish-farm, Slovenia
Authors: Marić, Saša 
Simonović, Predrag 
Razpet, Andrej
Keywords: Salmo trutta;fish farm;broodstock;genetic characterization;Slovenia
Issue Date: 2010
Rank: M23
Publisher: Hrvatsko Prirodoslovno Drustvo
Project: ON 143040
Journal: Periodicum Biologorum
Volume: 112
Start page: 145
End page: 148.
Abstract: 
Background and Purpose: Due to environmental and economic concerns, Bled fish-farm is interested in establishing broodstocks of native brown trout (Salmo trutta L.). Progeny would be reared and released into rivers managed by the Fishing Club Bled. In this study was performed genetic characterization of broodstock from Bled fish-farm in order to assess hybridization of native brown trout of Danubian phylogeographic lineage with trout of the allochtonous Atlantic lineage.
Material and Methods: DNA was isolated from fin clips of 20 males and 20 females from broodstock. PCR-RFLP technique was used for distinguishing between Atlantic and Danubian lineages on the basis of control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene (LDH).
Results: Results show a high percentage of allochthonous genetic markers especially among females and confirm hybridization between native and introduced brown trout.
Conclusions: Because of low percentage of native trout markers, Bled fish-farm decided to continue with collecting and genotyping fish to establish a broodstock with higher percentage of genetic markers characteristic for native trout of Danubian lineage.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1343
ISSN: 0031-5362
Appears in Collections:Journal Article

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