Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1255
Title: Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana guss. From Macedonia
Authors: Mileski, Ksenija 
Džamić, Ana 
Ćirić, Ana
Grujić, Slavica 
Ristić, M.
Matevski, V.
Marin, Petar 
Keywords: Abts;Antimicrobial activity;Apiaceae;Dpph;Echinophora sibthorpiana;Essential oil;Extracts;Flavonoids;Phenols
Issue Date: 20-Jan-2014
Journal: Archives of Biological Sciences
Abstract: 
This study was undertaken to determine the antioxidant and antimicrobial effect of essential oil and extracts of Echinophora sibthorpiana Guss. (fam. Apiaceae) collected in Macedonia. The chemical composition of E. sibthorpiana essential oil was characterized by the presence of methyl eugenol (60.40%), p-cymene (11.18%) and α-phellandrene (10.23%). The free radical scavenging activity of extracts and essential oil was evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results of the ABTS test showed that the most effective was the ethanol extract of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger antioxidant activity compared to hydroxyanisole, ascorbic acid and quercetin that were used in the DPPH and ABTS tests, respectively. The total phenolic and flavonoid concentrations in the extracts ranged between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The antimicrobial properties of the extracts and essential oil were investigated using a micro-well dilution technique against human pathogenic strains. The results were comparable with the effects of the positive controls, streptomycin and fluconazole. These findings indicate that E. sibthorpiana extracts and oil can be used in preventive treatments and as an alternative for synthetic preservatives.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1255
ISSN: 0354-4664
DOI: 10.2298/ABS1401401M
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