Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1166
Title: Targeting the superoxide/nitric oxide ratio by L-arginine and SOD mimic in diabetic rat skin
Authors: Jankovic, Aleksandra
Ferreri, Carla
Filipovic, Milos
Ivanovic-Burmazovic, Ivana
Stancic, Ana
Otasevic, Vesna
Korać, Aleksandra 
Buzadzic, Biljana
Korać, Bato 
Keywords: Diabetes;Nitric oxide;redox balance;Skin;SOD mimic;superoxide
Issue Date: 1-Nov-2016
Project: White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders 
COST Action CM1201
Journal: Free Radical Research
Abstract: 
Published by Informa UK Limited, trading as Taylor & Francis Group. Setting the correct ratio of superoxide anion (O2•-) and nitric oxide (•NO) radicals seems to be crucial in restoring disrupted redox signaling in diabetic skin and improvement of •NO physiological action for prevention and treatment of skin injuries in diabetes. In this study we examined the effects of L-arginine and manganese(II)-pentaazamacrocyclic superoxide dismutase (SOD) mimic–M40403 in diabetic rat skin. Following induction of diabetes by alloxan (blood glucose level ≥12 mMol l −1) non-diabetic and diabetic male Mill Hill hybrid hooded rats were divided into three subgroups: (i) control, and receiving: (ii) L-arginine, (iii) M40403. Treatment of diabetic animals started after diabetes induction and lasted for 7 days. Compared to control, lower cutaneous immuno-expression of endothelial NO synthase (eNOS), heme oxygenase 1 (HO1), manganese SOD (MnSOD) and glutathione peroxidase (GSH-Px), in parallel with increased NFE2-related factor 2 (Nrf2) and nitrotyrosine levels characterized diabetic skin. L-arginine and M40403 treatments normalized alloxan-induced increase in nitrotyrosine. This was accompanied by the improvement/restitution of eNOS and HO1 or MnSOD and GSH-Px protein expression levels in diabetic skin following L-arginine, i.e. SOD mimic treatments, respectively. The results indicate that L-arginine and M40403 stabilize redox balance in diabetic skin and suggest the underlying molecular mechanisms. Restitution of skin redox balance by L-arginine and M40403 may represent an effective strategy to ameliorate therapy of diabetic skin.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1166
ISSN: 1071-5762
DOI: 10.1080/10715762.2016.1232483
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