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Title: | Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes | Authors: | Golić, Igor Veličković, Ksenija Markelić, Milica Stancic, A. Jankovic, A. Vucetic, M. Otasevic, V. Buzadzic, B. Korać, Bato Korać, Aleksandra |
Keywords: | Brown adipocyte;Calcium;Endoplasmic reticulum;Mitochondrial dynamics | Issue Date: | 1-Jan-2014 | Rank: | M23 | Project: | White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders | Journal: | European Journal of Histochemistry | Abstract: | Mitochondria are key organelles maintaining cellular bioenergetics and integrity, and their regulation of [Ca2+]; homeostasis has been investigated in many cell types. We investigated the short-term Ca-SANDOZ® treatment on brown adipocyte mitochondria, using imaging and molecular biology techniques. Two-month- old male Wistar rats were divided into two groups: Ca-SANDOZ® drinking or tap water (control) drinking for three days. Alizarin Red S staining showed increased Ca2+ level in the brown adipocytes of treated rats, and potassium pyroantimonate staining localized electron- dense regions in the cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ® decreased mitochondrial number, but increased their size and mitochondrial cristae volume. Transmission electron microscopy revealed numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ® treated group compared to the control, and megamitochondria in some brown adipocytes. The Ca2+ diet affected mitochondrial fusion as mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and mitochondrial fission as dynamin related protein 1 (DRP1) was decreased. Confocal microscopy showed a higher colocalization rate between functional mitochondria and endoplasmic reticulum (ER). The level of uncoupling protein-1 (UCP1) was elevated, which was confirmed by immunohistochemistry and Western blot analysis. These results suggest that Ca-SANDOZ® stimulates mitochondrial fusion, increases mitochondrial-ER contacts and the thermogenic capacity of brown adipocytes. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/105 | ISSN: | 1121-760X | DOI: | 10.4081/ejh.2014.2377 |
Appears in Collections: | Journal Article |
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