Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/105
Title: Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes
Authors: Golić, Igor 
Veličković, Ksenija 
Markelić, Milica 
Stancic, A.
Jankovic, A.
Vucetic, M.
Otasevic, V.
Buzadzic, B.
Korać, Bato 
Korać, Aleksandra 
Keywords: Brown adipocyte;Calcium;Endoplasmic reticulum;Mitochondrial dynamics
Issue Date: 1-Jan-2014
Rank: M23
Project: White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders 
Journal: European Journal of Histochemistry
Abstract: 
Mitochondria are key organelles maintaining cellular bioenergetics and integrity, and their regulation of [Ca2+]; homeostasis has been investigated in many cell types. We investigated the short-term Ca-SANDOZ® treatment on brown adipocyte mitochondria, using imaging and molecular biology techniques. Two-month- old male Wistar rats were divided into two groups: Ca-SANDOZ® drinking or tap water (control) drinking for three days. Alizarin Red S staining showed increased Ca2+ level in the brown adipocytes of treated rats, and potassium pyroantimonate staining localized electron- dense regions in the cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ® decreased mitochondrial number, but increased their size and mitochondrial cristae volume. Transmission electron microscopy revealed numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ® treated group compared to the control, and megamitochondria in some brown adipocytes. The Ca2+ diet affected mitochondrial fusion as mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and mitochondrial fission as dynamin related protein 1 (DRP1) was decreased. Confocal microscopy showed a higher colocalization rate between functional mitochondria and endoplasmic reticulum (ER). The level of uncoupling protein-1 (UCP1) was elevated, which was confirmed by immunohistochemistry and Western blot analysis. These results suggest that Ca-SANDOZ® stimulates mitochondrial fusion, increases mitochondrial-ER contacts and the thermogenic capacity of brown adipocytes.
URI: https://biore.bio.bg.ac.rs/handle/123456789/105
ISSN: 1121-760X
DOI: 10.4081/ejh.2014.2377
Appears in Collections:Journal Article

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