Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/105
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dc.contributor.authorGolić, Igoren_US
dc.contributor.authorVeličković, Ksenijaen_US
dc.contributor.authorMarkelić, Milicaen_US
dc.contributor.authorStancic, A.en_US
dc.contributor.authorJankovic, A.en_US
dc.contributor.authorVucetic, M.en_US
dc.contributor.authorOtasevic, V.en_US
dc.contributor.authorBuzadzic, B.en_US
dc.contributor.authorKorać, Batoen_US
dc.contributor.authorKorać, Aleksandraen_US
dc.date.accessioned2019-06-19T21:00:37Z-
dc.date.available2019-06-19T21:00:37Z-
dc.date.issued2014-01-01-
dc.identifier.issn1121-760X-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/105-
dc.description.abstractMitochondria are key organelles maintaining cellular bioenergetics and integrity, and their regulation of [Ca2+]; homeostasis has been investigated in many cell types. We investigated the short-term Ca-SANDOZ® treatment on brown adipocyte mitochondria, using imaging and molecular biology techniques. Two-month- old male Wistar rats were divided into two groups: Ca-SANDOZ® drinking or tap water (control) drinking for three days. Alizarin Red S staining showed increased Ca2+ level in the brown adipocytes of treated rats, and potassium pyroantimonate staining localized electron- dense regions in the cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ® decreased mitochondrial number, but increased their size and mitochondrial cristae volume. Transmission electron microscopy revealed numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ® treated group compared to the control, and megamitochondria in some brown adipocytes. The Ca2+ diet affected mitochondrial fusion as mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and mitochondrial fission as dynamin related protein 1 (DRP1) was decreased. Confocal microscopy showed a higher colocalization rate between functional mitochondria and endoplasmic reticulum (ER). The level of uncoupling protein-1 (UCP1) was elevated, which was confirmed by immunohistochemistry and Western blot analysis. These results suggest that Ca-SANDOZ® stimulates mitochondrial fusion, increases mitochondrial-ER contacts and the thermogenic capacity of brown adipocytes.en_US
dc.language.isoenen_US
dc.relationWhite or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disordersen_US
dc.relation.ispartofEuropean Journal of Histochemistryen_US
dc.subjectBrown adipocyteen_US
dc.subjectCalciumen_US
dc.subjectEndoplasmic reticulumen_US
dc.subjectMitochondrial dynamicsen_US
dc.titleCalcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytesen_US
dc.typeArticleen_US
dc.identifier.doi10.4081/ejh.2014.2377-
dc.identifier.pmid25308841-
dc.identifier.scopus2-s2.0-84907579590-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/84907579590-
dc.description.rankM23en_US
dc.description.impact2.421en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0001-5944-5053-
crisitem.author.orcid0000-0002-4373-5483-
crisitem.author.orcid0000-0002-5444-7735-
crisitem.author.orcid0000-0001-5272-579X-
crisitem.author.orcid0000-0002-3044-9963-
crisitem.project.funderMESTD-
crisitem.project.grantno173055-
crisitem.project.fundingProgramBasic Research (BR or ON)-
crisitem.project.openAireinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173055-
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