Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/105
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Golić, Igor | en_US |
dc.contributor.author | Veličković, Ksenija | en_US |
dc.contributor.author | Markelić, Milica | en_US |
dc.contributor.author | Stancic, A. | en_US |
dc.contributor.author | Jankovic, A. | en_US |
dc.contributor.author | Vucetic, M. | en_US |
dc.contributor.author | Otasevic, V. | en_US |
dc.contributor.author | Buzadzic, B. | en_US |
dc.contributor.author | Korać, Bato | en_US |
dc.contributor.author | Korać, Aleksandra | en_US |
dc.date.accessioned | 2019-06-19T21:00:37Z | - |
dc.date.available | 2019-06-19T21:00:37Z | - |
dc.date.issued | 2014-01-01 | - |
dc.identifier.issn | 1121-760X | - |
dc.identifier.uri | https://biore.bio.bg.ac.rs/handle/123456789/105 | - |
dc.description.abstract | Mitochondria are key organelles maintaining cellular bioenergetics and integrity, and their regulation of [Ca2+]; homeostasis has been investigated in many cell types. We investigated the short-term Ca-SANDOZ® treatment on brown adipocyte mitochondria, using imaging and molecular biology techniques. Two-month- old male Wistar rats were divided into two groups: Ca-SANDOZ® drinking or tap water (control) drinking for three days. Alizarin Red S staining showed increased Ca2+ level in the brown adipocytes of treated rats, and potassium pyroantimonate staining localized electron- dense regions in the cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ® decreased mitochondrial number, but increased their size and mitochondrial cristae volume. Transmission electron microscopy revealed numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ® treated group compared to the control, and megamitochondria in some brown adipocytes. The Ca2+ diet affected mitochondrial fusion as mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and mitochondrial fission as dynamin related protein 1 (DRP1) was decreased. Confocal microscopy showed a higher colocalization rate between functional mitochondria and endoplasmic reticulum (ER). The level of uncoupling protein-1 (UCP1) was elevated, which was confirmed by immunohistochemistry and Western blot analysis. These results suggest that Ca-SANDOZ® stimulates mitochondrial fusion, increases mitochondrial-ER contacts and the thermogenic capacity of brown adipocytes. | en_US |
dc.language.iso | en | en_US |
dc.relation | White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders | en_US |
dc.relation.ispartof | European Journal of Histochemistry | en_US |
dc.subject | Brown adipocyte | en_US |
dc.subject | Calcium | en_US |
dc.subject | Endoplasmic reticulum | en_US |
dc.subject | Mitochondrial dynamics | en_US |
dc.title | Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.4081/ejh.2014.2377 | - |
dc.identifier.pmid | 25308841 | - |
dc.identifier.scopus | 2-s2.0-84907579590 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84907579590 | - |
dc.description.rank | M23 | en_US |
dc.description.impact | 2.421 | en_US |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | Article | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | Chair of Cell and Tissue Biology | - |
crisitem.author.dept | Chair of Cell and Tissue Biology | - |
crisitem.author.dept | Chair of Cell and Tissue Biology | - |
crisitem.author.dept | Chair of Cell and Tissue Biology | - |
crisitem.author.orcid | 0000-0001-5944-5053 | - |
crisitem.author.orcid | 0000-0002-4373-5483 | - |
crisitem.author.orcid | 0000-0002-5444-7735 | - |
crisitem.author.orcid | 0000-0001-5272-579X | - |
crisitem.author.orcid | 0000-0002-3044-9963 | - |
crisitem.project.funder | MESTD | - |
crisitem.project.grantno | 173055 | - |
crisitem.project.fundingProgram | Basic Research (BR or ON) | - |
crisitem.project.openAire | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173055 | - |
Appears in Collections: | Journal Article |
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