Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/104
Title: Effects of methimazole-induced hypothyroidism on immunohistochemical, stereomorphometric and some ultrastructural characteristics of pancreatic β-cells
Authors: Ukropina, Mirela 
Glišić, Radmila
Veličković, Ksenija 
Markelić, Milica 
Golić, Igor 
Čakić Milošević, Maja 
Koko, Vesna
Keywords: β-Cell granules;Hypothyroidism;Pancreas;PDX1
Issue Date: 30-Oct-2012
Rank: M23
Journal: Archives of Biological Sciences
Abstract: 
The function of pancreatic β-cells is to produce and secrete insulin, a crucial hormone in carbohydrate metabolism. The transcription factor PDX1 is required for insulin gene transcription and mature β-cell function. Since this factor is regulated by triiodothyronine, a disturbance in insulin biosynthesis and/or secretion is usually related to a deficiency of this hormone. In the present study, we used methods of immunohistochemistry, stereology and electron microscopy to explore the correlation between altered thyroid status and insulin synthesis/secretion in a model of methimazole-induced hypothyroidism in rats. In hypothyroid animals fewer functional PDX1-positive β-cells were detected in the islets of Langerhans, while insulin immunostaining was stronger. Stereological analysis of β-cell granules revealed more numerous immature insulin granules in hypothyroid rats. Taken together, these data suggest that the applied treatment caused impaired insulin synthesis and secretion. Rare cells with granules characteristic for both α- and β-cells observed in hypothyroid animals could provide functional compensation for diminished insulin synthesis.
URI: https://biore.bio.bg.ac.rs/handle/123456789/104
ISSN: 0354-4664
DOI: 10.2298/ABS1203943U
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