Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/730
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dc.contributor.authorStanić, Marinaen_US
dc.contributor.authorKrižak, Strahinjaen_US
dc.contributor.authorJovanović, Mirnaen_US
dc.contributor.authorPajić, Tanjaen_US
dc.contributor.authorĆirić, Anaen_US
dc.contributor.authorŽižić, Milanen_US
dc.contributor.authorZakrzewska, Joannaen_US
dc.contributor.authorCvetić Antić, Tijanaen_US
dc.contributor.authorTodorović, Natašaen_US
dc.contributor.authorŽivić, Miroslaven_US
dc.date.accessioned2019-07-08T21:31:55Z-
dc.date.available2019-07-08T21:31:55Z-
dc.date.issued2017-03-01-
dc.identifier.issn1350-0872-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/730-
dc.description.abstract© 2017 The Authors. Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 μM NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33±5 and 21±3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.en_US
dc.language.isoenen_US
dc.relation.ispartofMicrobiology (United Kingdom)en_US
dc.subjectATPen_US
dc.subjectCellular energy metabolismen_US
dc.subjectCellular respirationen_US
dc.subjectFungicideen_US
dc.subjectGrowth inhibitionen_US
dc.titleGrowth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolitesen_US
dc.typeArticleen_US
dc.identifier.doi10.1099/mic.0.000429-
dc.identifier.pmid28100310-
dc.identifier.scopus2-s2.0-85017022734-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85017022734-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.deptChair of Plant Physiology-
crisitem.author.orcid0000-0003-3794-7655-
crisitem.author.orcid0000-0003-1676-7508-
crisitem.author.orcid0000-0002-0314-5032-
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