Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/7260
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dc.contributor.authorMalešević, Anastasijaen_US
dc.contributor.authorTucović, Dinaen_US
dc.contributor.authorKulaš, Jelenaen_US
dc.contributor.authorMirkov, Ivanaen_US
dc.contributor.authorPopović, Dušankaen_US
dc.contributor.authorČakić-Milošević, Majaen_US
dc.contributor.authorPopov Aleksandrov, Aleksandraen_US
dc.date.accessioned2024-09-02T09:57:18Z-
dc.date.available2024-09-02T09:57:18Z-
dc.date.issued2024-08-08-
dc.identifier.issn1422-0067-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/7260-
dc.description.abstractThe skin is a direct target of the air pollutant benzo[a]pyrene (BaP). While its carcinogenic qualities are well-studied, the immunotoxicity of BaP after dermal exposure is less understood. This study examines the immunomodulatory effects of a 10-day epicutaneous BaP application, in environmentally/occupationally relevant doses, by analyzing ex vivo skin immune response (skin explant, epidermal cells and draining lymph node/DLN cell activity), alongside the skin's reaction to sensitization with experimental hapten dinitrochlorobenzene (DNCB). The results show that BaP application disrupts the structure of the epidermal layer and promotes immune cell infiltration in the dermis. BaP exposure led to oxidative stress in epidermal cells, characterized by decreased reduced glutathione and increased AHR and Cyp1A1 expression. Production and gene expression of proinflammatory cytokines (TNF, IL-1β) by epidermal cells decreased, while IL-10 response increased. Decreased spontaneous production of IFN-γ and IL-17, along with unchanged IL-10, was observed in DLC cells, whereas ConA-stimulated production of these cytokines was elevated. Local immunosuppression caused by BaP application seems to reduce the skin's response to an additional stimulus, evidenced by decreased effector activity of DLN cells three days after sensitization with DNCB. These findings provide new insight into the immunomodulatory effects and health risks associated with skin exposure to BaP.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofInternational journal of molecular sciencesen_US
dc.subjectdraining lymph nodes cell activityen_US
dc.subjectepicutaenous benzo[a]pyrene applicationen_US
dc.subjectepidermal cell activityen_US
dc.subjectratsen_US
dc.subjectskin explantsen_US
dc.titleImpact of Skin Exposure to Benzo[a]pyrene in Rat Model: Insights into Epidermal Cell Function and Draining Lymph Node Cell Responseen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.3390/ijms25168631-
dc.identifier.pmid39201318-
dc.description.rankM21en_US
dc.description.impact4.9en_US
dc.description.startpage8631en_US
dc.relation.issn1661-6596en_US
dc.description.volume25en_US
dc.description.issue16en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0002-2810-2135-
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