Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6713
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dc.contributor.authorKrstić Tomić, Tamaraen_US
dc.contributor.authorAtanasković, Ivaen_US
dc.contributor.authorNikolić, Ivanen_US
dc.contributor.authorJoković, Natašaen_US
dc.contributor.authorStević, Tatjanaen_US
dc.contributor.authorStanković, Slavišaen_US
dc.contributor.authorBerić, Tanjaen_US
dc.contributor.authorLozo, Jelenaen_US
dc.date.accessioned2023-11-27T09:14:27Z-
dc.date.available2023-11-27T09:14:27Z-
dc.date.issued2023-06-09-
dc.identifier.issn2076-2607-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/6713-
dc.description.abstractThe diversity of plant-associated bacteria is vast and can be determined by 16S rRNA gene metabarcoding. Fewer of them have plant-beneficial properties. To harness their benefits for plants, we must isolate them. This study aimed to check whether 16S rRNA gene metabarcoding has predictive power in identifying the majority of known bacteria with plant-beneficial traits that can be isolated from the sugar beet (Beta vulgaris L.) microbiome. Rhizosphere and phyllosphere samples collected during one season at different stages of plant development were analyzed. Bacteria were isolated on rich unselective media and plant-based media enriched with sugar beet leaves or rhizosphere extracts. The isolates were identified by sequencing the 16S rRNA gene and tested in vitro for their plant-beneficial properties (stimulation of germination; exopolysaccharide, siderophore, and HCN production; phosphate solubilization; and activity against sugar beet pathogens). The highest number of co-occurring beneficial traits was eight, found in isolates of five species: Acinetobacter calcoaceticus, Bacillus australimaris, B. pumilus, Enterobacter ludwiigi, and Pantoea ananatis. These species were not detected by metabarcoding and have not previously been described as plant-beneficial inhabitants of sugar beets. Thus, our findings point out the necessity of a culture-dependent microbiome analysis and advocate for low-nutrient plant-based media for high-yield isolation of plant-beneficial taxa with multiple beneficial traits. A culture-dependent and -independent approach is required for community diversity assessment. Still, isolation on plant-based media is the best approach to select isolates for potential use as biofertilizers and biopesticides in sugar beet cultivation.en_US
dc.language.isoenen_US
dc.relation.ispartofMicroorganismsen_US
dc.subject16S rRNA metabarcodingen_US
dc.subjectplant microbiomeen_US
dc.subjectplant-based mediaen_US
dc.subjectseasonal changesen_US
dc.subjectsugar beeten_US
dc.titleCulture-Dependent and Metabarcoding Characterization of the Sugar Beet (Beta vulgaris L.) Microbiome for High-Yield Isolation of Bacteria with Plant Growth-Promoting Traitsen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.3390/microorganisms11061538-
dc.identifier.pmid37375040-
dc.identifier.scopus2-s2.0-85163858462-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85163858462-
dc.description.rankM22en_US
dc.description.impact4,5en_US
dc.description.startpage1538en_US
dc.relation.issn2076-2607en_US
dc.description.volume11en_US
dc.description.issue6en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Microbiology-
crisitem.author.deptChair of Microbiology-
crisitem.author.deptChair of Microbiology-
crisitem.author.deptChair of Biochemistry and Molecular Biology-
crisitem.author.orcid0000-0001-7851-4050-
crisitem.author.orcid0000-0003-0527-8741-
crisitem.author.orcid0000-0002-4860-2225-
crisitem.author.orcid0000-0001-9888-5270-
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