Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6622
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dc.contributor.authorLuković, Bojanaen_US
dc.contributor.authorGajić, Inaen_US
dc.contributor.authorDimkić, Ivicaen_US
dc.contributor.authorKekić, Dusanen_US
dc.contributor.authorZornić, Sanjaen_US
dc.contributor.authorPozder, Tatjanaen_US
dc.contributor.authorRadisavljević, Svetlanaen_US
dc.contributor.authorOpavski, Natašaen_US
dc.contributor.authorKojić, Milanen_US
dc.contributor.authorRanin, Lazaren_US
dc.date.accessioned2023-11-23T10:09:10Z-
dc.date.available2023-11-23T10:09:10Z-
dc.date.issued2020-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/6622-
dc.description.abstractBackground The worldwide emergence and clonal spread of carbapenem-resistantAcinetobacter baumannii(CRAB) is of great concern. The aim of this nationwide study was to investigate the prevalence of CRAB isolates in Serbia and to characterize underlying resistance mechanisms and their genetic relatedness. Methods Non-redundant clinical samples obtained from hospitalized patients throughout Serbia were included in the prospective, observational, multicenter study conducted from January to June 2018. Samples were initially screened for the presence ofAcinetobacter baumannii-calcoaceticus(Acb) complex using conventional bacteriological techniques. Acb complexes recovered from clinical samples obtained from inpatients with confirmed bacterial infections were further evaluated for the presence ofA. baumannii. Identification to the species level was done by the detection of thebla(OXA-51)gene andrpoBgene sequence analysis. Susceptibility testing was done by disk diffusion and broth microdilution method. CRAB isolates were tested for the presence of acquired carbapenemases(bla(OXA-24-like),bla(OXA-23-like,)bla(OXA-58-like),bla(OXA-143-like),bla(IMP),bla(VIM),bla(GIM),bla(SPM),bla(SIM),bla(NDM)) by PCR. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results Acb complex was isolated in 280 out of 2401 clinical samples (11.6%). Overall,A. baumanniiwas identified in 237 out of 280 Acb complex (84.6%). CRAB prevalence was found to be 93.7% (237/222). The MIC50/MIC(90)for imipenem and meropenem were 8/ gt 32 mu g/mL and 16/ gt 32 mu g/mL, respectively. Although susceptibility was high for colistin (95.7%;n = 227) and tigecycline (75.1%;n = 178), ten isolates (4.3%) were classified as pandrug-resistant. The following carbapenemases-encoding genes were found: 98 (44.2%)bla(OXA-24-like), 76 (34.5%)bla(OXA-23-like), and 7 (3.2%)bla(NDM-1). PFGE analysis revealed six different clusters. MLST analysis identified three STs: ST2 (n = 13), ST492 (n = 14), and ST636 (n = 10). Obtained results evaluated that circulating CRAB clones in Serbia were as follows:bla(OXA66)/bla(OXA23)/ST2 (32.4%),bla(OXA66)/bla(OXA23)/bla(OXA72)/ST2 (2.7%),bla(OXA66)/bla(OXA72)/ST492 (37.8%), andbla(OXA66)/bla(OXA72)/ST636 (27.1%). Conclusion This study revealed extremely high proportions of carbapenem resistance amongA. baumanniiclinical isolates due to the emergence ofbla(OXA-72),bla(OXA-23), andbla(NDM-1)genes among CRAB isolates in Serbia and their clonal propagation.en_US
dc.language.isoenen_US
dc.publisherBMC, Londonen_US
dc.relation.ispartofAntimicrobial Resistance and Infection Controlen_US
dc.subjectST636;en_US
dc.subjectST492;en_US
dc.subjectCRAB;en_US
dc.subjectbla(OXA72);en_US
dc.subjectbla(OXA23);en_US
dc.subjectbla(NDM-1);en_US
dc.subjectAcinetobacter baumannii.en_US
dc.titleThe first nationwide multicenter study of Acinetobacter baumannii recovered in Serbia: emergence of OXA-72, OXA-23 and NDM-1-producing isolatesen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s13756-020-00769-8-
dc.description.rankM21en_US
dc.description.impact4.887en_US
dc.description.startpage1en_US
dc.relation.issn2047-2994en_US
dc.description.volume9en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Biochemistry and Molecular Biology-
crisitem.author.orcid0000-0002-0425-5938-
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