Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6443
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dc.contributor.authorAleksić, Marijaen_US
dc.contributor.authorGolić, Igoren_US
dc.contributor.authorJanković, Aleksandraen_US
dc.contributor.authorČvoro, Aleksandraen_US
dc.contributor.authorKorać, Aleksandraen_US
dc.date.accessioned2023-11-08T11:59:17Z-
dc.date.available2023-11-08T11:59:17Z-
dc.date.issued2023-05-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/6443-
dc.description.abstractWe previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.en_US
dc.language.isoenen_US
dc.relation.ispartofRoyal Society Open Scienceen_US
dc.subjectACOX1;en_US
dc.subjectACOX3;en_US
dc.subjectBrown adipocytes;en_US
dc.subjectHypothyroidism;en_US
dc.subjectPeroxisomes.en_US
dc.titleACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid ratsen_US
dc.typeArticleen_US
dc.identifier.doi10.1098/rsos.230109-
dc.description.rankM22en_US
dc.description.impact3.653en_US
dc.description.startpage230109en_US
dc.relation.issn2054-5703en_US
dc.description.volume10en_US
dc.description.issue5en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0003-0904-7043-
crisitem.author.orcid0000-0001-5944-5053-
crisitem.author.orcid0009-0007-5643-1634-
crisitem.author.orcid0000-0002-3044-9963-
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