Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/6436
DC Field | Value | Language |
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dc.contributor.author | Milton, Flora Aparecida | en_US |
dc.contributor.author | Čvoro, Aleksandra | en_US |
dc.contributor.author | Amato, Angelica A. | en_US |
dc.contributor.author | Sieglaff, Douglas H. | en_US |
dc.contributor.author | Filgueira, Carly S. | en_US |
dc.contributor.author | Arumanayagam, Anithachristy Sigamani | en_US |
dc.contributor.author | do Carmo Alves de Lima, Maria | en_US |
dc.contributor.author | Rocha Pitta, Ivan | en_US |
dc.contributor.author | de Assis Rocha Neves, Francisco | en_US |
dc.contributor.author | Webb, Paul | en_US |
dc.date.accessioned | 2023-11-08T11:58:19Z | - |
dc.date.available | 2023-11-08T11:58:19Z | - |
dc.date.issued | 2015-08-28 | - |
dc.identifier.uri | https://biore.bio.bg.ac.rs/handle/123456789/6436 | - |
dc.description.abstract | Thiazolidinediones (TZDs) are peroxisome proliferator-activated receptor gamma (PPARγ) agonists that improve insulin resistance but trigger side effects such as weight gain, edema, congestive heart failure and bone loss. GQ-16 is a PPARγ partial agonist that improves glucose tolerance and insulin sensitivity in mouse models of obesity and diabetes without inducing weight gain or edema. It is not clear whether GQ-16 acts as a partial agonist at all PPARγ target genes, or whether it displays gene-selective actions. To determine how GQ-16 influences PPARγ activity on a gene by gene basis, we compared effects of rosiglitazone (Rosi) and GQ-16 in mature 3T3-L1 adipocytes using microarray and qRT-PCR. Rosi changed expression of 1156 genes in 3T3-L1, but GQ-16 only changed 89 genes. GQ-16 generally showed weak effects upon Rosi induced genes, consistent with partial agonist actions, but a subset of modestly Rosi induced and strongly repressed genes displayed disproportionately strong GQ-16 responses. PPARγ partial agonists MLR24 and SR1664 also exhibit disproportionately strong effects on transcriptional repression. We conclude that GQ-16 displays a continuum of weak partial agonist effects but efficiently represses some negatively regulated PPARγ responsive genes. Strong repressive effects could contribute to physiologic actions of GQ-16. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Academic Press | en_US |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_US |
dc.subject | 3T3-L1; | en_US |
dc.subject | Gene expression; | en_US |
dc.subject | Partial agonist; | en_US |
dc.subject | Peroxisome proliferator activated receptor γ; | en_US |
dc.subject | Repression; | en_US |
dc.subject | Thiazolidinedione. | en_US |
dc.title | PPARγ partial agonist GQ-16strongly represses a subset of genes in 3T3-L1 adipocytes | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.bbrc.2015.07.011 | - |
dc.description.rank | M22 | en_US |
dc.description.impact | 2.559 | en_US |
dc.description.startpage | 718 | en_US |
dc.description.endpage | 723 | en_US |
dc.relation.issn | 0006-291X | en_US |
dc.description.volume | 464 | en_US |
dc.description.issue | 3 | en_US |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | Chair of Cell and Tissue Biology | - |
crisitem.author.orcid | 0009-0007-5643-1634 | - |
Appears in Collections: | Journal Article |
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