Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6327
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dc.contributor.authorStevanović, Katarinaen_US
dc.contributor.authorČepkenović, Bogdanaen_US
dc.contributor.authorKrižak, Strahinjaen_US
dc.contributor.authorŽivić, Miroslaven_US
dc.contributor.authorTodorović, Natašaen_US
dc.date.accessioned2023-10-12T07:46:54Z-
dc.date.available2023-10-12T07:46:54Z-
dc.date.issued2023-05-31-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/6327-
dc.description.abstractStudies of ion currents in filamentous fungi are a prerequisite for forming a complete understanding of their physiology. Cytoplasmic droplets (CDs), obtained from sporangiophores of Phycomyces blakesleeanus, are a model system that enables the characterization of ion currents in the native membrane, including the currents mediated by the channels not yet molecularly identified. Osmotically activated anionic current with outward rectification (ORIC) is a dominant current in the membrane of cytoplasmic droplets under the conditions of hypoosmotic stimulation. We have previously reported remarkable functional similarities of ORIC with the vertebrate volume regulated anionic current (VRAC), such as dose-dependent activation by osmotic difference, ion selectivity sequence, and time and voltage dependent profile of the current. Using the patch clamp method on the CD membrane, we further resolve VRAC-like ORIC characteristics in this paper. We examine the inhibition by extracellular ATP and carbenoxolone, the permeation of glutamate in presence of chloride, selectivity for nitrates, and activation by GTP, and we show its single channel behavior in excised membrane. We propose that ORIC is a functional counterpart of vertebrate VRAC in filamentous fungi, possibly with a similar essential role in anion efflux during cell volume regulation.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofJournal of Fungien_US
dc.subjectOsmotic stress;en_US
dc.subjectATP;en_US
dc.subjectNitrate;en_US
dc.subjectGlutamate;en_US
dc.subjectPatch-clamp;en_US
dc.subjectSingle channel current.en_US
dc.titleOsmotically Activated Anion Current of Phycomyces blakesleeanus-Filamentous Fungi Counterpart to Vertebrate Volume Regulated Anion Currenten_US
dc.typeArticleen_US
dc.identifier.doi10.3390/jof9060637-
dc.description.rankM21en_US
dc.description.impact4.7en_US
dc.description.startpage637en_US
dc.relation.issn2309-608Xen_US
dc.description.volume9en_US
dc.description.issue6en_US
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairetypeArticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.orcid0000-0001-9733-2367-
crisitem.author.orcid0000-0002-0314-5032-
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