Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4313
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dc.contributor.authorAleksić, Marijaen_US
dc.contributor.authorGolić, Igoren_US
dc.contributor.authorKalezić, Aen_US
dc.contributor.authorJanković, Aen_US
dc.contributor.authorKorać, Batoen_US
dc.contributor.authorKorać, Aleksandraen_US
dc.date.accessioned2021-10-22T15:04:45Z-
dc.date.available2021-10-22T15:04:45Z-
dc.date.issued2021-08-30-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/4313-
dc.description.abstractDespite peroxisomes being important partners of mitochondria by carrying out fatty acid oxidation in brown adipocytes, no clear evidence concerning peroxisome origin and way(s) of biogenesis exists. Herein we used methimazole-induced hypothyroidism for 7, 15, and 21 days to study peroxisomal remodeling and origin in rat brown adipocytes. We found that peroxisomes originated via both canonic, and de novo pathways. Each pathway operates in euthyroid control and over the course of hypothyroidism, in a time-dependent manner. Hypothyroidism increased the peroxisomal number by 1.8-, 3.6- and 5.8-fold on days 7, 15, and 21. Peroxisomal presence, their distribution, and their degree of maturation were heterogeneous in brown adipocytes in a Harlequin-like manner, reflecting differences in their origin. The canonic pathway, through numerous dumbbell-like and “pearls on strings” structures, supported by high levels of Pex11β and Drp1, prevailed on day 7. The de novo pathway of peroxisomal biogenesis started on day 15 and became dominant by day 21. The transition of peroxisomal biogenesis from canonic to the de novo pathway was driven by increased levels of Pex19, PMP70, Pex5S, and Pex26 and characterized by numerous tubular structures. Furthermore, specific peroxisomal origin from mitochondria, regardless of thyroid status, indicates their mutual regulation in rat brown adipocytes.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofCellsen_US
dc.subjectHypothyroidismen_US
dc.subjectBrown adipocyteen_US
dc.subjectPeroxisomeen_US
dc.subjectBiogenesis pathwayen_US
dc.subjectInter-organellar associationen_US
dc.titleHypothyroidism intensifies both canonic and the de novo pathway of peroxisomal biogenesis in rat brown adipocytes in a time-dependent manneren_US
dc.typeArticleen_US
dc.identifier.doi10.3390/cells10092248-
dc.description.rankM21en_US
dc.description.impact6,6en_US
dc.description.startpage2248en_US
dc.description.volume10en_US
dc.description.issue9en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0003-0904-7043-
crisitem.author.orcid0000-0001-5944-5053-
crisitem.author.orcid0000-0001-5272-579X-
crisitem.author.orcid0000-0002-3044-9963-
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