Biochemical analysis of gliadins of wheat Triticum durum

Abstract

Gliadins are one of the major protein fractions, which are deposited in endosperm protein bodies of grain. Composition of gliadin components in 21 durum wheat cultivars was separated by acid polyacrylamide gel electrophoresis. The obtained electrophoregrams by poliacrilamid gel electrophoresis were used for analysis of similarity of investigated durum wheat cultivars. Relative mobility and coloring intensity of components of gliadins (prolamins) were estimated and used for compilation of electrophoretic formulas for all cultivars. Electrophoretic formula for each cultivar was specific. Number of components per cultivar varied between 18 and 28. Variability of presence of some components indicates high polymorphysms of gliadins. Similarity among cultivars according to relative mobility of gliadins was from 9.52% to 61.53%.