Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/396
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dc.contributor.authorĐorđević, Markoen_US
dc.date.accessioned2019-07-01T20:46:57Z-
dc.date.available2019-07-01T20:46:57Z-
dc.date.issued2011-11-01-
dc.identifier.issn0021-9193-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/396-
dc.description.abstractClassical elements of σ 70 bacterial promoters include the -35 element ( -35TTGACA -30), the -10 element ( -12TATAAT -7), and the extended -10 element ( -15TG -14). Although the -35 element, the extended -10 element, and the upstream-most base in the -10 element ( -12T) interact with σ 70 in double-stranded DNA (dsDNA) form, the downstream bases in the -10 motif ( -11ATAAT -7) are responsible for σ 70-single-stranded DNA (ssDNA) interactions. In order to directly reflect this correspondence, an extension of the extended -10 element to a so-called -15 element ( -15TGnT -12) has been recently proposed. I investigated here the sequence specificity of the proposed -15 element and its relationship to other promoter elements. I found a previously undetected significant conservation of -13G and a high degeneracy at -15T. I therefore defined the -15 element as a degenerate motif, which, together with the conserved stretch of sequence between -15 and -12, allows treating this element analogously to -35 and -10 elements. Furthermore, the strength of the -15 element inversely correlates with the strengths of the -35 element and -10 element, whereas no such complementation between other promoter elements was found. Despite the direct involvement of -15 element in σ 70-dsDNA interactions, I found a significantly stronger tendency of this element to complement weak -10 elements that are involved in σ 70-ssDNA interactions. This finding is in contrast to the established view, according to which the -15 element provides a sufficient number of σ 70-dsDNA interactions, and suggests that the main parameter determining a functional promoter is the overall promoter strength. © 2011, American Society for Microbiology.en_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Bacteriologyen_US
dc.titleRedefining Escherichia coli σ <sup>70</sup> Promoter Elements: -15 Motif as a Complement of the -10 Motifen_US
dc.typeArticleen_US
dc.identifier.doi10.1128/JB.05947-11-
dc.identifier.pmid21908667-
dc.identifier.scopus2-s2.0-80655125448-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/80655125448-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
item.openairetypeArticle-
item.languageiso639-1en-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.orcid0000-0002-2903-3119-
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