Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3905
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dc.contributor.authorĆirković, Vladimiren_US
dc.contributor.authorUzelac, Aleksandraen_US
dc.contributor.authorMiličić, Draganaen_US
dc.contributor.authorKlun, Ivanaen_US
dc.contributor.authorĐurković-Đaković, Olgicaen_US
dc.date.accessioned2020-12-01T14:06:14Z-
dc.date.available2020-12-01T14:06:14Z-
dc.date.issued2019-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/3905-
dc.description.abstractToxoplasma gondii is one of the most successful parasites in the world. Its life form found in the environ-ment, where it may stay viable for a year or more, is the oocyst, excreted by members of the family Felidae as the definite host. During the past decades, several waterborne epidemics of toxoplasmosis worldwide indicated the importance of water as a source of T. gondii infection. In Serbia, detection of oocysts in wa-ter has not been previously performed and, therefore, there are no data regarding the presence of T. gondiiin Serbian rivers. To fill this gap, surface water samples (n=20) were collected from four major rivers and their tributaries throughout Serbia. The samples were concentrated by filtration and total DNA was extracted from the pellet using a commercial kit. Next, the samples were screened for T. gondii DNA by amplification of the 529 bp repeat fragment by conventional PCR. Three positive samples were detected and all three were confirmed by amplification of GRA6 and SAG2 genes. This first detection of T. gondii in river waters in Serbia suggests that the examined rivers are contaminated with this protozoan, which makes them a potential source of infection. In addition, this study has shown that the used PCR methodol-ogy can successfully detect T. gondii in water samples.en_US
dc.description.sponsorshipMinistry of Education, Science and Technological Develop-ment of the Republic of Serbiaen_US
dc.language.isootheren_US
dc.relationControl of infections by Apicomplexan pathogens: from novel drug targets to predictionen_US
dc.subjecttoxoplasmosisen_US
dc.subjectSurface wateren_US
dc.subjectOocystsen_US
dc.subjectDNAen_US
dc.subjectPCRen_US
dc.titleThe first detection of Toxoplasma gondii in rivers in Serbiaen_US
dc.typeConference Paperen_US
dc.relation.conferenceThird International Conference on Zoology, Zoonoses and Epidemiology, Bulgariaen_US
item.languageiso639-1other-
item.cerifentitytypePublications-
item.openairetypeConference Paper-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Morphology, Systematics and Phylogeny of Animals-
crisitem.author.orcid0000-0002-6034-5145-
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