Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/3905
DC Field | Value | Language |
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dc.contributor.author | Ćirković, Vladimir | en_US |
dc.contributor.author | Uzelac, Aleksandra | en_US |
dc.contributor.author | Miličić, Dragana | en_US |
dc.contributor.author | Klun, Ivana | en_US |
dc.contributor.author | Đurković-Đaković, Olgica | en_US |
dc.date.accessioned | 2020-12-01T14:06:14Z | - |
dc.date.available | 2020-12-01T14:06:14Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | https://biore.bio.bg.ac.rs/handle/123456789/3905 | - |
dc.description.abstract | Toxoplasma gondii is one of the most successful parasites in the world. Its life form found in the environ-ment, where it may stay viable for a year or more, is the oocyst, excreted by members of the family Felidae as the definite host. During the past decades, several waterborne epidemics of toxoplasmosis worldwide indicated the importance of water as a source of T. gondii infection. In Serbia, detection of oocysts in wa-ter has not been previously performed and, therefore, there are no data regarding the presence of T. gondiiin Serbian rivers. To fill this gap, surface water samples (n=20) were collected from four major rivers and their tributaries throughout Serbia. The samples were concentrated by filtration and total DNA was extracted from the pellet using a commercial kit. Next, the samples were screened for T. gondii DNA by amplification of the 529 bp repeat fragment by conventional PCR. Three positive samples were detected and all three were confirmed by amplification of GRA6 and SAG2 genes. This first detection of T. gondii in river waters in Serbia suggests that the examined rivers are contaminated with this protozoan, which makes them a potential source of infection. In addition, this study has shown that the used PCR methodol-ogy can successfully detect T. gondii in water samples. | en_US |
dc.description.sponsorship | Ministry of Education, Science and Technological Develop-ment of the Republic of Serbia | en_US |
dc.language.iso | other | en_US |
dc.relation | Control of infections by Apicomplexan pathogens: from novel drug targets to prediction | en_US |
dc.subject | toxoplasmosis | en_US |
dc.subject | Surface water | en_US |
dc.subject | Oocysts | en_US |
dc.subject | DNA | en_US |
dc.subject | PCR | en_US |
dc.title | The first detection of Toxoplasma gondii in rivers in Serbia | en_US |
dc.type | Conference Paper | en_US |
dc.relation.conference | Third International Conference on Zoology, Zoonoses and Epidemiology, Bulgaria | en_US |
item.languageiso639-1 | other | - |
item.cerifentitytype | Publications | - |
item.openairetype | Conference Paper | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | restricted | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | Chair of Morphology, Systematics and Phylogeny of Animals | - |
crisitem.author.orcid | 0000-0002-6034-5145 | - |
Appears in Collections: | Conference paper |
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File | Description | Size | Format | Existing users please |
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Suppl_15_15.pdf | 1.08 MB | Adobe PDF | Request a copy |
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