Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3862
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dc.contributor.authorBelić, Majaen_US
dc.contributor.authorZdravković-Korać, Snežanaen_US
dc.contributor.authorJanošević, Dušicaen_US
dc.contributor.authorSavić, Jelenaen_US
dc.contributor.authorTodorović, Slađanaen_US
dc.contributor.authorBanjac, Nevenaen_US
dc.contributor.authorMilojević, Jelenaen_US
dc.date.accessioned2020-11-30T12:29:50Z-
dc.date.available2020-11-30T12:29:50Z-
dc.date.issued2020-07-03-
dc.identifier.issn0167-6857-
dc.identifier.issn1573-5044-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/3862-
dc.description.abstractGibberellins (GAs) play a pivotal role in the induction of somatic embryogenesis from in vitro root apices of spinach plants. With the aim to understand the role of GAs in this process and to improve somatic embryo (SE) regeneration efficiency, the impact of light and GAs on SE initiation from the in vitro root apices was studied. The root sections were isolated from in vitro-grown SE-derived plants and placed on medium containing 20 μM α-naphthaleneacetic acid (NAA) and 0–10 μM GA3 or GA1, and cultivated under light conditions or in darkness. The most efficient SE regeneration response (100% regenerating SEs and 40.73 SEs per root apices) was achieved only in the presence of both light and GAs, with GA3 always exhibiting much stronger effect than GA1. Considering that light enhances GAs biosynthesis and the necessity of GAs for SE initiation, the expression levels of genes encoding the key enzymes involved in the final steps of GAs synthesis (SoGA20-ox1and SoGA3-ox1) and deactivation (SoGA2-ox1, SoGA2-ox2 and SoGA2-ox3) were analyzed. Light enhanced the expression of all five GA-ox genes, while exogenously supplied NAA + GA3 provoked downregulation of SoGA20-ox1 and SoGA3-ox1and upregulation of SoGA2ox-2 and SoGA2ox-3 expression. The expression of SoGA2ox-1 only slightly decreased. The results indicated the capability of isolated spinach roots to perceive the light and autonomously produce GAs. The expres-sion levels of genes encoding key enzymes involved in GA biosynthesis suggest that lower levels of GAs favor SE initiation.en_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Cell, Tissue and Organ Culture (PCTOC)en_US
dc.subjectGibberellin|Light|Regeneration|Root sections|Somatic embryogenesis|Spinachen_US
dc.titleGibberellins and light synergistically promote somatic embryogenesis from the in vitro apical root sections of spinachen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/s11240-020-01878-3-
dc.description.rankM22en_US
dc.description.impact2.730en_US
dc.description.startpage537en_US
dc.description.endpage548en_US
dc.description.volume142en_US
dc.description.issue3en_US
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Plant Physiology-
crisitem.author.orcid0000-0002-3541-4153-
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