Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/371
DC FieldValueLanguage
dc.contributor.authorKlimuk, Evgenyen_US
dc.contributor.authorBogdanova, Ekaterinaen_US
dc.contributor.authorNagornykh, Maxen_US
dc.contributor.authorRodić, Anđelaen_US
dc.contributor.authorĐorđević, Markoen_US
dc.contributor.authorMedvedeva, Sofiaen_US
dc.contributor.authorPavlova, Olgaen_US
dc.contributor.authorSeverinov, Konstantinen_US
dc.date.accessioned2019-07-01T13:36:42Z-
dc.date.available2019-07-01T13:36:42Z-
dc.date.issued2018-01-01-
dc.identifier.issn0305-1048-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/371-
dc.description.abstract© 2018 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research. C-proteins control restriction-modification (R-M) systems' genes transcription to ensure sufficient levels of restriction endonuclease to allow protection from foreign DNA while avoiding itsmodification by excess methyltransferase. Here, we characterize transcription regulation in C-protein dependent R-M system Kpn2I. The Kpn2I restriction endonuclease gene is transcribed from a constitutive, weak promoter, which, atypically, is C-protein independent. Kpn2I C-protein (C.Kpn2I) binds upstream of the strong methyltransferase gene promoter and inhibits it, likely by preventing the interaction of the RNA polymerase sigma subunit with the-35 consensus element. Diminished transcription from the methyltransferase promoter increases transcription from overlapping divergent C-protein gene promoters. All known C-proteins affect transcription initiation from R-M genes promoters. Uniquely, the C.Kpn2I binding site is located within the coding region of its gene. C.Kpn2I acts as a roadblock stalling elongating RNA polymerase and decreasing production of full-length C.Kpn2I mRNA. Mathematical modeling shows that this unusual mode of regulation leads to the same dynamics of accumulation of R-M gene transcripts as observed in systems where C-proteins act at transcription initiation stage only. Bioinformatics analyses suggest that transcription regulation through binding of C.Kpn2I-like proteins within the coding regions of their genes may be widespread.en_US
dc.language.isoenen_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.titleController protein of restriction-modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblocken_US
dc.typeArticleen_US
dc.identifier.doi10.1093/nar/gky880-
dc.identifier.pmid30295835-
dc.identifier.scopus2-s2.0-85056589277-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85056589277-
dc.description.rankM21a-
dc.description.impact16.971-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.deptChair of General Physiology and Biophysics-
crisitem.author.orcid0000-0003-2872-9066-
crisitem.author.orcid0000-0002-2903-3119-
Appears in Collections:Journal Article
Files in This Item:
Show simple item record

SCOPUSTM   
Citations

10
checked on Nov 18, 2024

Page view(s)

13
checked on Nov 21, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.