Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/350
Title: Prognostic value of the DNMTs mRNA expression and genetic polymorphisms on the clinical outcome in oral cancer patients
Authors: Supic, Gordana
Kozomara, Ruzica
Zeljić, Katarina 
Jovic, Nebojsa
Magic, Zvonko
Keywords: DNMT1;DNMT3A;DNMT3B;mRNA;Oral cancer;Overall survival;Overexpression;Polymorphism;Relapse-free survival
Issue Date: 1-Jan-2017
Journal: Clinical Oral Investigations
Abstract: 
© 2016, Springer-Verlag Berlin Heidelberg. Objectives: Although the importance of the epigenetic changes in tumors, including oral squamous cell carcinomas (OSCCs), is now becoming apparent, the mechanisms that trigger or cause aberrant DNA methylation in cancer are still unrevealed. DNA methylation is regulated by a family of enzymes, DNA methyltransferases (DNMTs). DNMT gene expression analysis, as well as genetic polymorphisms, has not been previously evaluated in OSCC. Materials and methods: In 65 OSCC patients, SYBR Green real-time PCR method was assessed for relative quantification of DNMT1, DNMT3A, and DNMT3B mRNAs, normalized to TATA-binding protein (TBP) mRNA. The expression levels of all three genes were dichotomized as high or low, with a twofold change of normalized mRNA expression used as the cutoff value. Polymorphisms in DNMT1 (rs2228612) and DNMT3B (rs406193) were analyzed in 99 OSCCs by TaqMan SNPs genotyping assays. Results: DNMT1, DNMT3A, and DNMT3B were overexpressed in 36.9, 26, and 23 % of the OSCC patients, respectively. DNMT1 overexpression was significantly associated with the overall survival, p = 0.029, and relapse-free survival of OSCC patients, p = 0.003. Patients with DNMT1 overexpression, as an independent prognostic factor, had a 2.385 times higher risk to relapse than those with lower expression. The DNMT1 A201G gene polymorphism was associated with a reduced overall survival in OSCC patients, p = 0.036. Conclusions: Our results suggest that DNMT1 could play an important role in modulating OSCC patient survival. Clinical relevance: DNMT gene expression could be a potential prognostic marker that might lead to an improvement in diagnosis, prognosis, and prospective use of epigenetic-targeted therapy of OSCC.
URI: https://biore.bio.bg.ac.rs/handle/123456789/350
ISSN: 1432-6981
DOI: 10.1007/s00784-016-1772-9
Appears in Collections:Journal Article

Show full item record

SCOPUSTM   
Citations

29
checked on Nov 4, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.