Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3168
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dc.contributor.authorTimotijevic, Gordana S.en_US
dc.contributor.authorRadović, Svetlana R.en_US
dc.contributor.authorMaksimovic, Vesna R.en_US
dc.date.accessioned2019-11-04T13:57:21Z-
dc.date.available2019-11-04T13:57:21Z-
dc.date.issued2003-03-26-
dc.identifier.issn0021-8561-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/3168-
dc.description.abstractThe pepstatin A sensitive acidic proteolytic activity of total protein extracts of buckwheat seeds has been analyzed in developing, mature, and germinating seeds by activity measurements as well as by electrophoretic and immunochemical techniques. Immunoblot analysis using cross-reactive antibodies raised against barley phytepsin suggested that specific proteolytic activity could be attributed to a 47 kDa heterodimeric polypeptide, composed of two subunits: 31 and 16 kDa polypeptides. The analysis of time course expression revealed that the 47 kDa heterodimer accumulated during seed maturation starting from 12 days after pollination and was also present at the beginning of germination. Milk-clotting activity of this proteinase was also indicated.en_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_US
dc.subject"in-gel" assayen_US
dc.subjectAspartic proteinaseen_US
dc.subjectBuckwheaten_US
dc.subjectImmunodetectionen_US
dc.subjectMilk-clotting activityen_US
dc.titleCharacterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum Moench) seedsen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/jf026043b-
dc.identifier.pmid12643680-
dc.identifier.scopus2-s2.0-0242669343-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/0242669343-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Biochemistry and Molecular Biology-
crisitem.author.orcid0000-0002-7546-6468-
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