Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/159
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dc.contributor.authorKalezic, Andjelikaen_US
dc.contributor.authorMacanovic, Biljanaen_US
dc.contributor.authorGaralejic, Elianaen_US
dc.contributor.authorKorać, Aleksandraen_US
dc.contributor.authorOtasevic, Vesnaen_US
dc.contributor.authorKorać, Batoen_US
dc.date.accessioned2019-06-25T15:10:07Z-
dc.date.available2019-06-25T15:10:07Z-
dc.date.issued2018-08-01-
dc.identifier.issn0009-2797-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/159-
dc.description.abstractNitric oxide (NO) is a signaling molecule responsible for initiation of molecular events that influence sperm functionality in a concentration-dependent manner. It is still not fully understood how seminal plasma NO contributes to sperm pathologies. The aim of this study was to elucidate whether and how NO is implicated in etiology of different sperm abnormalities. To this end we determine NO, nitrite and 3-nitrotyrosine (3-NT) content in seminal plasma of infertile men with specific pathologies (terato-, oligoterato- and oligoasthenoteratospermia) and relate it to infertile normospermic samples. To gain further understanding of NO metabolism in seminal plasma we determine protein expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). Here we show that NO, nitrite and 3-NT levels in seminal plasma of men with suboptimal semen parameters are significantly lower compared to normospermic men. An increase in protein expression of eNOS and no change in protein expression of iNOS is observed in men with sperm pathologies. Association of seminal plasma 3-NT level with functional sperm parameters is observed - positive correlation with sperm count, motility and morphology in normospermia, teratospermia and oligoteratospermia, as well as negative correlation with sperm morphology and motility in oligoasthenoteratospermia. Present study revealed that suboptimal seminal plasma NO content is found in all examined sperm pathologies. This result unequivocally shows the importance of NO for sperm function and involvement of suboptimal NO level in etiology of sperm abnormalities. Lower seminal plasma 3-NT level and its significant association with sperm parameters, found in pathologies, strongly indicates that protein nitration is important for spermatozoa function and that failure to establish this post-translational protein modification might be involved in etiology of sperm abnormalities. According to our results, NO measurement can discriminate infertile men with sperm pathologies from infertile normospermic men but is not indicative of a specific type of sperm pathology.en_US
dc.language.isoenen_US
dc.relationReactive oxygen and nitrogen species functions in reproduction: possible pharmacological tools to treat human infertilityen_US
dc.relation.ispartofChemico-Biological Interactionsen_US
dc.subject3-Nitrotyrosineen_US
dc.subjectMale infertilityen_US
dc.subjectNitric oxideen_US
dc.subjectNitriteen_US
dc.subjectSeminal plasmaen_US
dc.subjectSperm parametersen_US
dc.titleLevel of NO/nitrite and 3-nitrotyrosine in seminal plasma of infertile men: Correlation with sperm number, motility and morphologyen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.cbi.2018.07.002-
dc.identifier.pmid29983398-
dc.identifier.scopus2-s2.0-85049430526-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85049430526-
dc.description.rankM21-
dc.description.impact5.192-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0002-3044-9963-
crisitem.author.orcid0000-0001-5272-579X-
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