Participation of rec genes of Escherichia coli K12 in W-reactivation of UV-irradiated phage λ

Abstract

The effect of the recombinational deficiency on W-reactivation of UV-damaged phage λ was explored. In this paper we show that W-reactivation is reduced by the recB21 and recF143 mutations after bleomycin (BM) and UV treatment. Combination of these mutations in the recB21recF143 double mutant blocks W-reactivation completely after BM induction, but leaves residual W-reactivation ability after UV-irradiation, which is abolished by the introduction of uvrB deficiency (Δ(uvrB-chlA)). W-reactivation has been rendered constitutive in recB21C22sbcB15, but the efficiency of reactivation remained virtually constant over the range of BM and UV doses, indicating the role of the RecBC(D) enzyme in W-reactivation. © 1990.