Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/141
Title: Expression patterns of mitochondrial OXPHOS components, mitofusin 1 and dynamin-related protein 1 are associated with human embryo fragmentation
Authors: Otasevic, Vesna
Surlan, Lela
Vucetic, Milica
Tulic, Ivan
Buzadzic, Biljana
Stancic, Ana
Jankovic, Aleksandra
Veličković, Ksenija 
Golić, Igor 
Markelić, Milica 
Korać, Aleksandra 
Korać, Bato 
Keywords: in vitro fertilisation;membrane potential;mitochondrial DNA
Issue Date: 1-Jan-2016
Rank: M22
Project: Reactive oxygen and nitrogen species functions in reproduction: possible pharmacological tools to treat human infertility 
Journal: Reproduction, Fertility and Development
Abstract: 
Developmental dysfunction in embryos, such as a lethal level of fragmentation, is assumed to be mitochondrial in origin. This study investigated the molecular basis of mitochondrial impairment in embryo fragmentation. Transcription patterns of factors that determine mitochondrial functionality: (i) components of the oxidative phosphorylation (OXPHOS) - complex I, cytochrome b, complex IV and ATP synthase; (ii) mitochondrial membrane potential (MMP); (iii) mitochondrial DNA (mtDNA) content and (iv) proteins involved in mitochondrial dynamics, mitofusin 1 (Mfn1) and dynamin related protein 1 (Drp1) were examined in six-cells Day 3 non-fragmented (control), low-fragmented (LF) and high-fragmented (HF) human embryos. Gene expression of mitochondria-encoded components of complex I and IV, cytochrome b and mtDNA were increased in HF embryos compared with control and LF embryos. In LF embryos, expression of these molecules was decreased compared with control and HF embryos. Both classes of fragmented embryos had decreased MMP compared with control. LF embryos had increased gene expression of Mfn1 accompanied by decreased expression of Drp1, while HF embryos had decreased Mfn1 expression but increased Drp1 expression. The study revealed that each improper transcriptional (in)activation of mitochondria-encoded components of the OXPHOS during early in vitro embryo development is associated with a decrease in MMP and with embryo fragmentation. The results also showed the importance of mitochondrial dynamics in fragmentation, at least in the extent of this process.
URI: https://biore.bio.bg.ac.rs/handle/123456789/141
ISSN: 1031-3613
DOI: 10.1071/RD13415
Appears in Collections:Journal Article

Show full item record

SCOPUSTM   
Citations

9
checked on Nov 24, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.